D1538

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D1538

Sigma

 

1,4-Bis(acryloyl)piperazine

for electrophoresis

Synonym:1,4-Di(acryloyl)piperazine, N,N′-Bis(acryloyl)piperazine, BAP, PDA, Piperazine diacrylamide
CAS Number:6342-17-2
Linear Formula:C10H14N2O2
Molecular Weight:194.23
Beilstein Registry Number:745431
MDL number:MFCD00077661
PubChem Substance ID:24893477

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Description

ApplicationPDA is used as a cross-linker for polyacrylamide gels to reduce background on silver-stained gels. PDA has also been shown to increase resolution and increase gel strength.

Properties

gradefor electrophoresis
mp91.5-93.5 °C(lit.)

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Gloves
Hazard CodesXi
Risk Statements36/37/38
Safety Statements26-36
WGK Germany3

References

referenceHochstrasser, D.F., et al., Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining. Anal. Biochem. 173, 412-423, (1988) Abstract
 Hochstrasser, D.F., Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal. Biochem. 173, 424, (1988) Abstract
 Artoni, G., et al., Fractionation techniques in a hydro-organic enviroment. II. Acryloyl-morpholine polymers as a matrix for electrophoresis in hydro-organic solvents. Anal. Biochem. 137, 420-428, (1984) Abstract
BeilsteinBeil. 23,V,1,48